H.J. “Rick” Mascagni, Jr. and Brenda Tubana
Introduction
Nitrogen (N) fertilization is a critical cultural practice required for producing maximum corn yield. Many factors, including soil type and crop management systems, determine optimum N rates. Nitrogen is typically knifed-in soon after the crop has emerged and an adequate stand established. Growers often times split N fertilizer applications as part of their management system or, in some cases, due to uncontrollable factors such as excessive or lack of rainfall, may produce soil conditions conducive to N fertilizer loss through denitrification and/or inefficient plant N uptake. If N is topdressed with a fertilizer containing urea losses may occur due to volatization, which depends to a large extent on climatic and soil factors. If irrigated or rainfall occurs (0.5 inch or greater) within about three days, the fertilize is incorporated and no or minimal volatization losses will occur Sometimes N applications are delayed or omitted due to inclement weather, while at other times, growers apply the recommended N rate for an expected yield potential. However, as the crop develops yield potential may be higher than expected and additional N may be required. In each of the above situations the question arises, how late can N fertilizer be applied and be effective? The fertilizer N source is also an important component of an effective fertility program. Products are also available such as urease inhibitors (i.e., Agrotain) that minimize urea volatization losses for 7 to 10 days. The objective of this trial was to evaluate N applications, N sources, and an urease inhibitor at different growth stages on a Mississippi River silt loam.
Procedures
A field experiment was conducted in 2011 on Commerce silt loam at the Northeast Research Station near St. Joseph to evaluate the influence of N rate, timing, and fertilizer source on corn yield and N fertilizer use efficiency (NFUE). Early-season N rates were injected at about the 3-leaf growth stage (April 16) as 30-0-0-2 solution (UAN) at N rates of 0, 120, 150, 180, and 210 lb N/acre. Urea, with and without Agrotain (3 qts/ton urea), was also hand-broadcast at the rate of 120 lb N/acre at the 3-leaf growth stage. For the early-season N rate of 120 lb/acre using 30-0-0-2, supplemental N rates of 30 and 60 lb/acre were applied at about the 12-leaf (May 23) and early-silk growth stages (June 7). Urea, with and without Agrotain, was hand-broadcast and 30-0-0-2, with and without Agrotain, was hand-dribbled (to simulate a dribble application) at the 12-leaf and early-silk applications. There were a total of 23 treatments (see Table 2). REV® 28HR20™ was planted on March 24 at 32,000 seed/acre. Cotton was the previous crop and all LSU AgCenter recommended cultural practices were followed.
The experimental design was a randomized complete block with four replications. Grain yield, yield components, plant N, seed N, NFUE, and remote sensing data were determined. Grain yield was determined by machine harvest from the two middle rows of four-row plots and reported at 15.5% moisture. Yield components, seed weight (g/100 seed) and ear size (seed/ear) were also determined from the two middle rows. Ear-leaf samples were collected at the early- silk growth stage to determine the influence of treatments on the N status of the plant. Seed samples were also collected at harvest. Total N was determined in the plant tissue and harvested seed by the LSU AgCenter’s Soil and Plant Testing Lab. Seed-N uptake (lb N/acre) was calculated by multiplying seed-N concentration by grain yield. NFUE was calculated using the following formula: (seed-N uptake for a given N rate – seed-N uptake for the no-N control) / N rate. Remote sensing data using a SPAD meter were also determined at the 3- and 12-leaf growth stages. Statistical analyses were performed using the GLM procedure of SAS using a probability level of 0.10.
Results and Discussion
Rainfall was extremely low in May with a only a total of 4.9 inches in May and June in this dryland trial (Table 1). However, overall yields were extremely good averaging over 150 bu/acre (Table 2).
At early-season, urea, urea + Agrotain, and UAN were compared at the 120 lb N/acre rate. Yield response had the following rank: UAN = urea + Agrotain > urea (Table 2). Evidently, there was some N loss due to volatization for the urea fertilizer. There was a 10 day interval between application and the first rainfall event. For the late N applications at the 12-leaf growth stage and early silk, both the 30 and 60 lb N/acre rates increased yields across sources. Yields tended to be a little higher for the early silk compared to 12-leaf applications. There were 11 and 2 day intervals between application and rainfall for the 12-leaf and early-silk applications, respectively. There was a yield response to urea + Agrotain for the 30 lb N/acre late application at the 12-leaf growth stage. When comparing equivalent N rates applied either once early season or split between early season and 12 leaf or early-silk growth stages, yields were similar. The treatment influence on kernel weight and ear size (kernel number) are shown in Table 2.
Plant and seed N data are presented in Table 3. Leaf N, seed N, seed N uptake, and NFUE had the following rank for the early-season N treatments: UAN>urea+Agrotain>urea. Similar to yield responses, there were only small differences between the 12-leaf and early-silk late N applications for each N trait. Nitrogen fertilizer use efficiency (NFUE) was extremely high, ranging from 0.36 to 0.78 (Table 3). There were no differences in NFUE between the single and split applications, when comparing equivalent N rates. SPAD readings reflected treatment effects similar to yield responses (Table 4).
Table 1. Rainfall in St. Joseph, 2011.
Month |
Rainfall
|
|
inches
|
|
|
March |
8.3
|
April |
3.0
|
May |
0.9
|
June |
4.0
|
July |
4.4
|
August |
1.3
|
Table 2. Influence of N fertility treatments on corn yield and yield components on Commerce silt loam, 2011.
|
|
|
N rate
|
|
|
|
|
|
|
|
ESN1 rate |
ESN
source2
|
12-leaf
|
Early silk
|
Late N
source
|
Total N
applied
|
Yield
|
Ears
|
Kernel
weight
|
Kernels
|
lb/a |
|
———lb/a——
|
|
lb/a
|
bu/a
|
no/a
|
g/100
|
no/ear
|
|
|
|
|
|
|
|
|
|
|
0 |
–
|
–
|
–
|
–
|
0
|
39.6
|
31,390
|
31.7
|
124
|
120 |
Urea
|
–
|
–
|
–
|
120
|
116.7
|
32,700
|
32.0
|
293
|
120 |
Urea + Ag
|
–
|
–
|
–
|
120
|
141.9
|
32,700
|
33.4
|
329
|
120 |
UAN
|
–
|
–
|
–
|
120
|
145.8
|
33,350
|
34.4
|
365
|
|
|
|
|
|
|
|
|
|
|
120 |
UAN
|
30
|
–
|
Urea
|
150
|
160.7
|
33,350
|
34.7
|
327
|
120 |
UAN
|
30
|
–
|
Urea+Ag
|
150
|
169.4
|
32,700
|
34.1
|
386
|
120 |
UAN
|
30
|
–
|
UAN
|
150
|
165.8
|
30,740
|
34.7
|
423
|
120 |
UAN
|
30
|
–
|
UAN+Ag
|
150
|
165.6
|
32,700
|
36.0
|
385
|
Average |
|
|
|
|
165.4
|
32,370
|
34.9
|
380
|
|
|
|
|
|
|
|
|
|
|
120 |
UAN
|
60
|
–
|
Urea
|
180
|
170.0
|
32,050
|
35.2
|
386
|
120 |
UAN
|
60
|
–
|
Urea+Ag
|
180
|
176.8
|
34,010
|
36.2
|
397
|
120 |
UAN
|
60
|
–
|
UAN
|
180
|
160.3
|
33,350
|
35.2
|
357
|
120 |
UAN
|
60
|
–
|
UAN+Ag
|
180
|
166.2
|
32,700
|
35.1
|
378
|
Average |
|
|
|
|
168.3
|
33,030
|
35.4
|
380
|
|
|
|
|
|
|
|
|
|
|
120 |
UAN
|
–
|
30
|
Urea
|
150
|
168.5
|
34,660
|
35.1
|
361
|
120 |
UAN
|
–
|
30
|
Urea+Ag
|
150
|
151.8
|
33,350
|
34.9
|
368
|
120 |
UAN
|
–
|
30
|
UAN
|
150
|
168.7
|
32,700
|
35.7
|
389
|
120 |
UAN
|
–
|
30
|
UAN+Ag
|
150
|
168.0
|
33,350
|
34.3
|
386
|
Average |
|
|
|
|
164.3
|
33,520
|
35.0
|
376
|
|
|
|
|
|
|
|
|
|
|
120 |
UAN
|
–
|
60
|
Urea
|
180
|
177.0
|
32,700
|
35.6
|
403
|
120 |
UAN
|
–
|
60
|
Urea+Ag
|
180
|
172.8
|
34,010
|
34.4
|
383
|
120 |
UAN
|
–
|
60
|
UAN
|
180
|
166.5
|
33,350
|
34.1
|
381
|
120 |
UAN
|
–
|
60
|
UAN+Ag
|
180
|
170.0
|
32,700
|
35.1
|
393
|
Average |
|
|
|
|
171.6
|
33,190
|
34.8
|
390
|
|
|
|
|
|
|
|
|
|
|
150 |
UAN
|
–
|
–
|
–
|
150
|
166.2
|
34,010
|
34.3
|
374
|
180 |
UAN
|
–
|
–
|
–
|
180
|
169.8
|
30,740
|
33.9
|
432
|
210 |
UAN
|
–
|
–
|
–
|
210
|
178.8
|
34,010
|
36.7
|
380
|
|
|
|
|
|
|
|
|
|
|
LSD (0.10): |
|
|
|
|
14.7
|
NS3
|
2.7
|
53
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
1ESN, early-season N injected at about 3-leaf growth stage.
2Ag = Agrotain; UAN = 30-0-0-2;
3NS = Non-significant at the 0.10 probability level
Table 3. Influence of N fertility treatments on N nutrition of corn on Commerce silt loam, 2011.
|
|
|
N rate
|
|
|
|
|
|
|
ESN1 rate |
ESN
source2
|
12-leaf
|
Early silk
|
Late N
source
|
Total N
applied
|
Leaf N
|
Seed N
|
Seed N
uptake
|
NFUE3
|
lb/a |
|
———lb/a——
|
|
lb/a
|
%
|
%
|
lb N/a
|
|
|
|
|
|
|
|
|
|
|
|
0 |
–
|
–
|
–
|
–
|
0
|
1.18
|
1.28
|
39.7
|
–
|
120 |
Urea
|
–
|
–
|
–
|
120
|
1.57
|
1.22
|
82.0
|
0.36
|
120 |
Urea + Ag
|
–
|
–
|
–
|
120
|
1.86
|
1.38
|
107.9
|
0.57
|
120 |
UAN
|
–
|
–
|
–
|
120
|
2.24
|
1.43
|
133.0
|
0.78
|
|
|
|
|
|
|
|
|
|
|
120 |
UAN
|
30
|
–
|
Urea
|
150
|
2.30
|
1.40
|
116.9
|
0.52
|
120 |
UAN
|
30
|
–
|
Urea+Ag
|
150
|
2.23
|
1.43
|
135.0
|
0.64
|
120 |
UAN
|
30
|
–
|
UAN
|
150
|
2.26
|
1.44
|
141.7
|
0.68
|
120 |
UAN
|
30
|
–
|
UAN+Ag
|
150
|
2.32
|
1.45
|
143.5
|
0.69
|
Average |
|
|
|
|
2.28
|
1.43
|
134.3
|
0.63
|
|
|
|
|
|
|
|
|
|
|
120 |
UAN
|
60
|
–
|
Urea
|
180
|
2.32
|
1.50
|
142.4
|
0.57
|
120 |
UAN
|
60
|
–
|
Urea+Ag
|
180
|
2.27
|
1.55
|
166.4
|
0.71
|
120 |
UAN
|
60
|
–
|
UAN
|
180
|
2.15
|
1.50
|
138.1
|
0.55
|
120 |
UAN
|
60
|
–
|
UAN+Ag
|
180
|
2.36
|
1.52
|
145.4
|
0.59
|
Average |
|
|
|
|
2.28
|
1.52
|
148.1
|
0.61
|
|
|
|
|
|
|
|
|
|
|
|
|
120 |
UAN
|
–
|
30
|
Urea
|
150
|
–
|
1.40
|
135.0
|
0.64
|
|
120 |
UAN
|
–
|
30
|
Urea+Ag
|
150
|
–
|
1.44
|
136.1
|
0.64
|
|
120 |
UAN
|
–
|
30
|
UAN
|
150
|
–
|
1.48
|
146.9
|
0.72
|
|
120 |
UAN
|
–
|
30
|
UAN+Ag
|
150
|
–
|
1.48
|
143.7
|
0.70
|
|
Average |
|
|
|
|
–
|
1.45
|
140.4
|
0.68
|
|
|
|
|
|
|
|
|
|
|
|
|
120 |
UAN
|
–
|
60
|
Urea
|
180
|
–
|
1.47
|
151.7
|
0.63
|
|
120 |
UAN
|
–
|
60
|
Urea+Ag
|
180
|
–
|
1.53
|
149.8
|
0.61
|
|
120 |
UAN
|
–
|
60
|
UAN
|
180
|
–
|
1.47
|
139.1
|
0.56
|
|
120 |
UAN
|
–
|
60
|
UAN+Ag
|
180
|
–
|
1.45
|
142.4
|
0.58
|
|
Average |
|
|
|
|
–
|
1.48
|
145.8
|
0.60
|
|
|
|
|
|
|
|
|
|
|
|
|
150 |
UAN
|
–
|
–
|
–
|
150
|
2.28
|
1.45
|
139.3
|
0.67
|
|
180 |
UAN
|
–
|
–
|
–
|
180
|
2.50
|
1.49
|
146.9
|
0.60
|
|
210 |
UAN
|
–
|
–
|
–
|
210
|
2.48
|
1.48
|
154.4
|
0.55
|
|
|
|
|
|
|
|
|
|
|
|
|
LSD (0.10): |
|
|
|
|
|
0.16
|
0.10
|
21.7
|
0.14
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
1ESN, early-season N injected at about 3-leaf growth stage.
2Ag = Agrotain; UAN = 30-0-0-2;
3NFUE = N fertilizer use efficiency
Table 4. Influence of N fertility treatments on SPAD readings taken early season and at 12-leaf growth stage on Commerce silt loam, 2011.
|
|
|
N Fertilizer Source
|
|
|
N rate1 |
Total N
|
Urea
|
Urea + Agrotain
|
UAN2
|
UAN + Agrotain
|
Average
|
lb/acre |
|
———————————SPAD Readings ————————————- |
|
|
|
|
|
|
|
|
|
|
Early-Season N Application
|
|
|
|
|
|
|
|
|
120 |
120
|
38.5
|
44.0
|
46.9
|
–
|
43.1
|
150 |
150
|
–
|
–
|
48.5
|
–
|
48.5
|
180 |
180
|
–
|
–
|
51.1
|
–
|
51.1
|
210 |
210
|
–
|
–
|
52.4
|
–
|
52.4
|
|
|
|
|
|
|
|
|
|
|
12-leaf Growth Stage N Application |
|
|
|
|
|
|
30 |
150
|
49.2
|
46.4
|
50.0
|
50.4
|
49.0
|
60 |
180
|
50.1
|
52.2
|
48.4
|
51.2
|
50.5
|
Average |
|
49.7
|
49.3
|
49.2
|
50.8
|
|
|
|
|
|
|
|
|
LSD (0.10): |
|
|
|
3.8
|
|
|
|
|
|
|
|
|
|
|
|
|
1N rate applied early-season (3-leaf) and 12-leaf growth stage
2UAN = 30-0-0-2 fertilize solution